Studies were made of the production of venom in honeybees (Apis mellifera) of various ages. The venom glands were isolated, fixed with alcohol and dried over CaCl2. Bees 5 days of age produced 51 [gamma] dried venom (0.153 mg. original liquid venom); bees of 10 days 79 [gamma] (0.237 mg. original liquid venom); and bees 25 days old 110 [gamma] (0.33 mg.). When young bees were isolated in a celluloid cage in the hive and fed on sugar solns. exclusively, it was found that at the age of 10 days only 18.5 [gamma] of dried venom (0.056 mg. original dried venom) were formed. A diet devoid of pollen is undesirable for the production of bee venom.