Localization and Purification of a Secretory Protein from the Esophageal Glands ofMeloidogyne incognitawith a Monoclonal Antibody

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Abstract
Strong labeling of secretory granules in dorsal esophageal glands of preparasitic second-stage juveniles and adult females of Meloidogyne incognita by affinity-purified monoclonal antibody 6D4 was observed by indirect immunofluroescence microscopy. This antibody also reacted weakly with secretory granules in the subventral esophageal glands of preparasitic juveniles. Postembedding immunogold labeling of ultrathin sections of juvenile esophageal regions revealed that the antigen was segregated around an electron-transparent core in the maxtix of secretory granules in the subventral glands. In the dorsal gland ampulla of adult females, specific gold labeling was localized principally in clumps in the cytoplasm adjacent to secretory granules. The antigen also was detected by immunofluorescence microscopy in stylet secretions of adult females. This secretory component was immunoaffinity purified and appeared to be a large-molecular-weight (Mr > 212,000) glycoprotein as indicated by its slow electrophoretic migration in 7.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and positive staining with periodic acid-Schiff reagent.