Assessment of estrogen receptor--histone interactions.
- 1 May 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (5) , 2874-2878
- https://doi.org/10.1073/pnas.78.5.2874
Abstract
Several different in vitro binding assays show that the estrogen receptor from rabbit uterus interacts selectively with purified histones from calf thymus. The estrogen receptor binds strongly to histones H2B and H2A, moderately to histones H3 and H4 and poorly to histone H1. In the presence of histones H2B or H2A, the position at which the estrogen receptor focuses in an isoelectric gradient is shifted to a more basic zone. Kinetic experiments show that, if histone H2B is bound to a DNA, the estrogen receptor dissociates more slowly from that DNA. The portion of the estrogen receptor molecule required for binding to histone H2B is relatively stable to tryptic digestion; the portion of the receptor molecule responsible for DNA binding is promptly lost during limited tryptic digestion. These in vitro findings suggest that the mechanism by which the estrogen receptor selectively alters gene expression may involve specific contacts with histone molecules.Keywords
This publication has 41 references indexed in Scilit:
- A method for the purification of histone fraction F3 by affinity chromatographyArchives of Biochemistry and Biophysics, 1973
- The interaction of uterine estrogen receptors with DNAJournal of Steroid Biochemistry, 1972
- Interactions of histones with corticosteroid hormonesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The use of protamine to study [6,7-3H]-oestradiol-17β binding in rat uterusBiochemical Journal, 1970
- The properties of a nuclear acidic protein fraction that binds [6,7−3H]oestradiol-17βBiochemical Journal, 1969
- Interaction of steroid hormones with histones in vitroBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- High resolution acrylamide gel electrophoresis of histonesArchives of Biochemistry and Biophysics, 1969
- Structural specificity of the steroids interacting with calf thymus histonesBiochemical and Biophysical Research Communications, 1967
- Studies on histones. 7. Preparative methods for histone fractions from calf thymusBiochemical Journal, 1964