Abstract
Myotis tricolor was monoestrous and monotocous. The oestrous cycle was characterized by a prolonged period of oestrus between mid-April and mid-September and a short period of anoestrus between the termination of lactation and the onset of pro-oestrus. The female reproductive cycle was characterized by copulation in mid-April and ovulation and fertilization in mid-September. The gestation period was estimated to have been 63 days and parturition occurred between mid-November and mid-December. Parturition was followed by a six-week period of lactation. It is assumed that between mid-April and mid-September the female stored spermatozoa in the uterine horns, a reproductive strategy previously reported for north-temperate members of this genus.

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