Modification of Yeast Uricase with Polyethylene Glycol: Disappearance of Binding Ability towards Anti-Uricase Serum
- 1 July 1979
- journal article
- research article
- Published by S. Karger AG in Enzyme
- Vol. 24 (4) , 261-264
- https://doi.org/10.1159/000458668
Abstract
Uricase from Candida utilis was modified with activated polyethylene glycol (2-0-methoxypolyethylene glycol-4,6-dichloro-s-triazine) of molecular weight of 5,000 daltons. The modification of 43% of the total amino groups in the uricase molecule gave rise to a complete loss of the binding ability towards anti-uricase serum from rabbit. This modified uricase retained 15% of the enzymic activity of non-modified uricase.Keywords
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