Analysis of feline plasma proteins by immunoelectrophoresis and polyacrylamide gel electrophoresis.

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Abstract
Twenty-one feline plasma proteins, albumin, .alpha.1-acid glycoprotein, .alpha.1-antitrypsin, anti-thrombin III, ceruloplasmin, haptoglobin, .alpha.2-macroglobulin, hemopexin, transferrin, apolipoprotein (apo) A-I, apo A-II, apo B, apo C-I, apo C-III, immunoglobulin (Ig) G, IgA, IgM, complement (C) 3, C4, plasminogen, and fibrinogen, were purified and identified in immunoelectrophoretogram and polyacrylamide gel electrophoretogram. Methods used were polyacrylamide gel electrophoresis (PAGE), SDS-PAGE, agarose electrophoresis, immunoelectrophoresis (IEP), crossed immunoelectrophoresis (CIE), and electro transfer blotting (ETB) for the identification. The positions of 21 feline plasma proteins on immunoelectrophoretogram or polyacrylamide gel electrophoretogram were determined.