Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA
Open Access
- 1 January 2010
- journal article
- Published by Springer Nature in BMC Biotechnology
- Vol. 10 (1) , 8
- https://doi.org/10.1186/1472-6750-10-8
Abstract
Efficient expression systems exist for antibody (Ab) molecules, which allow for characterization of large numbers of individual Ab variants. In contrast, such expression systems have been lacking for soluble T cell receptors (TCRs). Attempts to generate bacterial systems have generally resulted in low yields and material which is prone to aggregation and proteolysis. Here we present an optimized periplasmic bacterial expression system for soluble single chain (sc) TCRs.Keywords
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