A method for linking yeast artificial chromosomes
Open Access
- 1 November 1996
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 24 (21) , 4192-4196
- https://doi.org/10.1093/nar/24.21.4192
Abstract
A method for linking any standard yeast artificial chromosomes (YAC) is described. YACs are introduced into the same cell and joined by mitotic recombination between the vector arms and the homologous sequence in a linking vector; several YACs can be recombined sequentially. The linking vectors also contain the β-galactosidase gene as an expression reporter in mammalian cells.Keywords
This publication has 14 references indexed in Scilit:
- Analysis of extrachromosomal structures containing human centromeric alphoid satellite DNA sequences in mouse cellsChromosoma, 1996
- Addition of functional human telomeres to YACsHuman Molecular Genetics, 1994
- Yeast kar1 Mutants Provide an Effective Method for YAC Transfer to New HostsGenomics, 1994
- A first-generation physical map of the human genomeNature, 1993
- A YAC contig across the fragile X site defines the region of fragilityNucleic Acids Research, 1991
- The human HPRT gene on a yeast artificial chromosome is functional when transferred to mouse cells by cell fusionGenomics, 1991
- Rapid assessment of S. cerevisiae mating type by PCRTrends in Genetics, 1990
- Transformation of yeast spheroplasts without cell fusionAnalytical Biochemistry, 1987
- Cloning of Large Segments of Exogenous DNA into Yeast by Means of Artificial Chromosome VectorsScience, 1987
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1984