Pullulanase-Amylase Complex Enzyme fromBacillus subtilis
- 1 January 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 51 (1) , 9-16
- https://doi.org/10.1080/00021369.1987.10867996
Abstract
A novel pullulanase-amylase complex enzyme, which hydrolyzes pullulan into maltotriose as well as starch into maltose and maltotriose as the main products, was found in the culture filtrate of a strain of Bacillus subtilis newly isolated from soil. The enzyme was purified to almost complete homogeneity by means of calcium phosphate gel adsorption, DEAE-Sepharose column chromatography and Bio-gel A-1.5m filtration. The optimum pH of the pullulanase activity was observed at around 7.0 to 7.5, with a discernible shoulder around pH 5.0. While the optimum pH of the amylase activity was 6 ~ 7. The optimum temperatures of the pullulanase and amylase activities were about 60°C and about 50°C, respectively. The molecular weight was estimated to be about 450,000 by the gel filtration method. The enzyme could be used for the production of glucose from starch with glucoamylase and the production of a new type of syrup containing a relatively high amount of maltotriose, 50 ~ 55%, from starch.This publication has 5 references indexed in Scilit:
- Production of Maltohexaose by α-Amylase fromBacillus circulansG-6Agricultural and Biological Chemistry, 1982
- Production of Maltose by Pullulanase and β-AmylasePublished by Springer Nature ,1981
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- Estimation of the molecular weights of proteins by Sephadex gel-filtrationBiochemical Journal, 1964
- ACTION OF AMYLO-1,6-GLUCOSIDASE AND PHOSPHORYLASE ON GLYCOGEN AND AMYLOPECTINJournal of Biological Chemistry, 1950