The Primary Structure of the Major Parvalbumin from Hake Muscle

Abstract
With the aim of establishing a starting point for the determination of the complete amino acid sequence of the major parvalbumin from hake muscle, the peptides resulting from the tryptic digestion of the S‐sulfo‐derivative and of the performic‐acid‐oxidized protein were examined.A total of 20 peptides were thus obtained in sufficient quantity and in a state of purity adequate for further studies. Their complete or partial sequence is reported.When due account is taken of obvious overlaps, the total of these peptides fully accounts for the composition of the whole molecule with the exception of one alanine residue, and for the number of peptides expected from the presence of 12 lysines and 1 arginine in this parvalbumin.

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