SYNTHESIS OF THE FRACTION I ANTIGENIC PROTEIN BYPASTEURELLA PESTIS

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Abstract
The temperature dependent synthesis of the fraction I antigen of P. pestis was studied with intact resting cells and with sonically disrupted cells. Cells, initially grown at 26 C and resuspended in phosphate buffer, required incubation at 37 C, aeration and casein hydrolysate for detectable antigen synthesis. Sonically disrupted cells were capable of fraction I synthesis only if the debris and supernatant fractions were recombined. The active nondialyzable substance in the soluble fraction was resistant to trypsin, ribonuclease, and desoxyribonuclease. A sensitive, specific hemagglutination -inhibition technique was employed for assay of antigen formation.