Über die pH- und Temperatur-Abhängigkeit des thermischen Zerfalls von Poliovirus-Teilchen in Nucleinsäure und leere Proteinhüllen

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Abstract
The intact virus particles of highly purified, concentrated suspensions of poliovirus have been disintegrated into free nucleic acid and empty protein shells (78 S protein) by moderate heat treatment at various temperatures and pH values. The kinetics of this degradation has been followed by ultracentrifugal analysis. With increasing temperature between 35° and 50 °C and with increasing pH of the suspension medium between 7 and 8, the rate of degradation increased. For any particular conditions the initial rate was not maintained, and after a certain time there was little further degradation if conditions remained unaltered. Some particles with the sedimentation characteristics of poliovirus were resistant to disintegration under the same conditions which led to the breakdown of the bulk of the virus. The proportion of this “stable fraction” varied within a wide range from one preparation to another and decreased with increasing temperature and with increasing pʜ.

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