Recombinant Human-Mouse Chimeric Monoclonal Antibody Specific for Human Adenocarcinoma Associated Antigen
- 1 February 1991
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 10 (1) , 1-9
- https://doi.org/10.1089/hyb.1991.10.1
Abstract
We have recently described a class-switched (IgM to IgG1) human-mouse chimeric antibody. In the present study, a human-mouse chimeric antibody specific for human adenocarcinoma-assocated atigen YH206 antigen was costrcted by fusing murine varible region genes (Vκ and VH) to human cnstant region genes (γ1,κ) The murine variable domain genes were isolated from a funconal murine hybridoma cell line, YH206, which secreted IgM monoclonal antibody specific for YH206 antgen. The fusion gens of heavy and light chins were introduced into the immunoglobulin non-prodcing mouse myeloma cell line X63-Ag8.653 by electroporation. We obtained transformants which secreted class-switched human-mouse chimeric antibodies specific for YH206 antigen. A dot immunobinding assay demonstrated that the class-switched chimeric antibody retained the ability to bind to the YH206 antigen.Keywords
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