L-asparaginase production by Erwinia aroideae.

  • 1 July 1969
    • journal article
    • Vol. 18  (1) , 64-7
Abstract
Maximum yields of 1,250 IU (international unit)/g (dry weight of cells) of L-asparaginase were obtained in 8 hr from Erwinia aroideae NRRL B-138. Partial purification and concentration of the extracted L-asparaginase yielded a preparation with an activity of 275 IU/ml. Only one L-asparaginase was present as determined by electrophoresis, and the enzyme exhibited a pH optimum of 7.5 and a K(m) of 3 x 10(-3) M.