EFFECTS OF HEMORRHAGIC-SHOCK ON NUCLEAR-DNA

  • 1 January 1979
    • journal article
    • research article
    • Vol. 85  (3) , 297-302
Abstract
Rats subjected to hemorrhagic shock had liver DNA labeled with 3H-thymidine. Reversible hemorrhagic shock in 9 rats with a blood pressure of 40 mm Hg for 30 min resulted in no DNA cleavage. Twenty-seven animals were subjected to severe hemorrhagic shock. Blood pressures of 40 mm Hg were maintained until 50% of the shed blood was reinfused in 11 animals and until 70% of the shed blood was reinfused in 16 animals. Severe hemorrhage caused DNA cleavage; 45.1% and 52.1% was small MW DNA, as compared with 9.5% and 10.8% in the preshocked animals (P < 0.001). DNA was not repaired 1.5 h after animals were resuscitated after severe hemorrhagic shock.