Genetic Variability in the G Protein Gene of Group A and B Respiratory Syncytial Viruses from India
- 1 September 2006
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 44 (9) , 3055-3064
- https://doi.org/10.1128/jcm.00187-06
Abstract
Respiratory syncytial virus (RSV) is the most commonly identified viral agent of acute respiratory tract infection (ARI) of young children and causes repeat infections throughout life. Limited data are available on the molecular epidemiology of RSV from developing countries, including India. This study reports on the genetic variability in the glycoprotein G gene among RSV isolates from India. Reverse transcription-PCR for a region of the RSV G protein gene was done with nasopharyngeal aspirates (NPAs) collected in a prospective longitudinal study in two rural villages near Delhi and from children with ARI seen in an urban hospital. Nucleotide sequence comparisons among 48 samples demonstrated a higher prevalence of group A (77%) than group B (23%) RSV isolates. The level of genetic variability was higher among the group A viruses (up to 14%) than among the group B viruses (up to 2%). Phylogenetic analysis revealed that both the GA2 and GA5 group A RSV genotypes were prevalent during the 2002-2003 season and that genotype GA5 was predominant in the 2003-2004 season, whereas during the 2004-2005 season both genotype GA5 and genotype BA, a newly identified group B genotype, cocirculated in almost equal proportions. Comparison of the nonsynonymous mutation-to-synonymous mutation ratios (dN/dS) revealed greater evidence for selective pressure between the GA2 and GA5 genotypes (dN/dS, 1.78) than within the genotypes (dN/dS, 0.69). These are among the first molecular analyses of RSV strains from the second most populous country in the world and will be useful for comparisons to candidate vaccine strains.Keywords
This publication has 54 references indexed in Scilit:
- A phylogenetic study of human respiratory syncytial viruses group A and B strains isolated in two cities in Japan from 1980–2002Journal of Medical Virology, 2005
- Respiratory Syncytial Virus Epidemiology in a Birth Cohort from Kilifi District, Kenya: Infection during the First Year of LifeThe Journal of Infectious Diseases, 2004
- Genotypes of respiratory syncytial virus group B identified in UruguayArchiv für die gesamte Virusforschung, 2004
- Molecular epidemiology of respiratory syncytial virus in Kilifi district, KenyaJournal of Medical Virology, 2004
- Nosocomial outbreak of respiratory syncytial virus subgroup B variants with the 60 nucleotides-duplicated G protein geneJournal of Medical Virology, 2004
- Genetic diversity of respiratory syncytial virus isolated during an epidemic period from children of northeastern BrazilJournal of Medical Virology, 2004
- Major changes in the G protein of human respiratory syncytial virus isolates introduced by a duplication of 60 nucleotidesJournal of General Virology, 2003
- Antigenic and genetic variability of human respiratory syncytial viruses (group A) isolated in Uruguay and Argentina: 1993–2001Journal of Medical Virology, 2003
- Genetic Variability among Group A and B Respiratory Syncytial Virus Isolates from a Large Referral Hospital in New Delhi, IndiaJournal of Clinical Microbiology, 2003
- Molecular epidemiology of respiratory syncytial virus: A review of the use of reverse transcription‐polymerase chain reaction in the analysis of genetic variabilityElectrophoresis, 1995