Evans Blue Staining Method for Detection of Proteins on Polyacrylamide Gels with Rhodamine B
- 1 May 1994
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 27 (7) , 1265-1275
- https://doi.org/10.1080/00032719408006367
Abstract
A new staining method employing a mixed dye technique is simple, rapid, and sensitive. Solutions of Evans blue (EB, 0.01 %) and rhodamine B (RB, 0.5 %) were mixed up with 1:1 volume ratio. This mixed dye staining method detects as little as 10 ng of bovine serum albumine (BSA). EB is used for protein staining based on its structural similarity to Coomassie brilliant blue R–250 (CB). RB acts as a supplementary agent which inhibits the binding of EB to gel matrix, and so increases the staining effect of EB and enables to achieve cutting down staining/destaining times.Keywords
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