Identification of Candidate Biomarker Proteins Released by Human Endometrial and Cervical Cancer Cells Using Two-Dimensional Liquid Chromatography/Tandem Mass Spectrometry
- 25 May 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 6 (7) , 2615-2622
- https://doi.org/10.1021/pr0700798
Abstract
Candidate biomarker proteins, including chaperonin 10 and pyruvate kinase, previously discovered and identified using mass-tagging reagents with multidimensional liquid chromatography and tandem mass spectrometry (DeSouza, L.; et al. J. Proteome Res. 2005, 4, 377−386) have been identified in serum-free media of cultured endometrial cancer (KLE and HEC-1-A) and cervical cancer (HeLa) cells. These and other cancer-associated proteins were released by the cultured cells within 24 h of growth. A total of 203 proteins from the KLE cells, 86 from HEC-1-A, and 161 from HeLa are reported. Keywords: Endometrial cancer • Cell released proteins • Candidate biomarkers • Two-dimensional liquid chromatography/tandem mass spectrometryKeywords
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