Abstract
Alcaligenes defragrans strains denitrify on monoterpenes with an unsaturated hydrocarbon structure. A new cometabolic reaction, the formation of isoterpinolene from isolimonene, was detected in cultures that grew on a monoterpene. The biotransformation of isolimonene, a monocyclic monoterpene with a sp3-hybridized C1 atom of the menthane skeleton, contrasts with the complete mineralization of monoterpenes with a sp2-hybridized C1 atom. This selectivity indicates a demand for a sp2-hybridized C1 atom as structural property for monoterpenes that can be oxidized by A. defragrans.

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