Molecular Size Diversity of Citrate Synthases from Pseudomonas Species

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Abstract
Two forms of citrate synthase (EC 4.1.3.7) have been found in several species of Pseudomonas, a ''large'' form (Mr .simeq. 250000) which is generally inhibited by NADH and reactivated by AMP, and a ''small'' form (Mr .simeq. 100000) which is insensitive to these nucleotide effectors. Other species of Pseudomonas were found to contain either the ''large'' or the ''small'' form. Gel filtration and ion-exchange with the technique of fast protein liquid chromatography were used to resolve the enzymes. Where both citrate synthases were present, there did not appear to be an equilibrium between the two forms. The results reveal a new and complex diversity of citrate synthase within the genus Pseudomonas.

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