The AAUAAA sequence is required both for cleavage and for polyadenylation of simian virus 40 pre-mRNA in vitro.
Open Access
- 1 July 1986
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 6 (7) , 2317-2323
- https://doi.org/10.1128/mcb.6.7.2317
Abstract
The sequence AAUAAA is found near the polyadenylation site of eucaryotic mRNAs. This sequence is required for accurate and efficient cleavage and polyadenylation of pre-mRNAs in vivo. In this study we show that synthetic simian virus 40 late pre-mRNAs are cleaved and polyadenylated in vitro in a HeLa cell nuclear extract, and that cleavage in vitro is abolished by each of four different single-base changes in AAUAAA. In this same extract, precleaved RNAs (RNAs with 3' termini at the polyadenylation site) are efficiently polyadenylated. This in vitro polyadenylation reaction also requires the AAUAAA sequence.This publication has 31 references indexed in Scilit:
- Accurate cleavage and polyadenylation of exogenous RNA substrateCell, 1985
- A sequence downstream of AAUAAA is required for rabbit β-globin mRNA 3′-end formationNature, 1984
- Are U4 small nuclear ribonucleoproteins involved in polyadenylation?Nature, 1984
- Site-specific polyadenylation in a cell-free reactionCell, 1984
- Most κ immunoglobulin mRNA in human lymphocytes is homologous to a small family of germ-line V genesNature, 1984
- Post-transcriptional processing of simian virus 40 late transcripts in injected frog oocytesJournal of Molecular Biology, 1983
- Transcription and RNA processing by the DNA tumour virusesNature, 1980
- Steps in the processing of Ad2 mRNA: Poly(A)+ Nuclear sequences are conserved and poly(A) addition precedes splicingCell, 1978
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976