EFFECTS OF VERAPAMIL ON DAUNOMYCIN CELLULAR RETENTION AND CYTO-TOXICITY IN P388 LEUKEMIC-CELLS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (5) , 1743-1747
Abstract
Digitized video microscopy was utilized to investigate the influence of verapamil, a Ca channel blocker, on the in vitro effects of daunomycin in [mouse leukemia] P388 sensitive and resistant sublines. Verapamil enhanced the uptake of daunomycin and its cytotoxicity in both sublines, but the effect was more pronounced in the resistant cells. Flow cytometry showed a pronounced accumulation of resistant cells at G2-M when treated with daunomycin in the presence of verapamil, with no effects observed in the absence of verapamil. Analysis of individual resistant cells using the digitized video fluorescence microscopy technique demonstrated that the influence of verapamil on daunomycin uptake affected all cells and was not restricted to certain subpopulations of cells.