Cloning of a hemolysin gene from Leptospira interrogans serovar hardjo
- 1 August 1989
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 57 (8) , 2588-90
- https://doi.org/10.1128/iai.57.8.2588-2590.1989
Abstract
A DNA fragment encoding both hemolysin and sphingomyelinase C activity was cloned from the pathogenic bacterium Leptospira interrogans serovar hardjo. Initial clones were obtained by screening a genomic library in EMBL3 for hemolytic activity. Both hemolytic and sphingomyelinase C activities were coded for by a 3.9-kilobase BamHI fragment. The hemolysin was expressed from its own promoter in Escherichia coli K-12. Similar DNA sequences were also present in the serovars tarassovi and ballum.Keywords
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