Recombinant‐DNA‐derived bovine growth hormone from Escherichia coli
- 3 March 1987
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 163 (2) , 323-330
- https://doi.org/10.1111/j.1432-1033.1987.tb10803.x
Abstract
Bacterially synthesized, recombinant‐DNA‐derived bovine growth hormone (r‐bGH), prepared as described in the preceding paper in this journal, has been characterized in comparison with pituitary bovine growth hormone (pit‐bGH). The characterization criteria include sodium dodecyl sulfate/polyacrylamide gel electrophoresis, automated N‐terminal sequence analysis, amino acid composition, isoelectric focusing, reverse‐phase high‐performance liquid chromatography, ultraviolet absorbance, analysis for free protein thiol, sizing by gel filtration, circular dichroism, radioimmunoassay and biological activity in the hypophysectomized rat weight‐gain assay. In every respect the r‐bGH appears to be virtually identical to pit‐bGH.This publication has 38 references indexed in Scilit:
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