The tegument surface membranes of the human blood parasite Schistosoma mansoni: A proteomic analysis after differential extraction
Top Cited Papers
- 2 March 2006
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 6 (5) , 1471-1482
- https://doi.org/10.1002/pmic.200500368
Abstract
The blood fluke Schistosoma mansoni can live for years in the hepatic portal system of its human host and so must possess very effective mechanisms of immune evasion. The key to understanding how these operate lies in defining the molecular organisation of the exposed parasite surface. The adult worm is covered by a syncytial tegument, bounded externally by a plasma membrane and overlain by a laminate secretion, the membranocalyx. In order to determine the protein composition of this surface, the membranes were detached using a freeze/thaw technique and enriched by sucrose density gradient centrifugation. The resulting preparation was sequentially extracted with three reagents of increasing solubilising power. The extracts were separated by 2‐DE and their protein constituents were identified by MS/MS, yielding predominantly cytosolic, cytoskeletal and membrane‐associated proteins, respectively. After extraction, the final pellet containing membrane‐spanning proteins was processed by liquid chromatographic techniques before MS. Transporters for sugars, amino acids, ions and other solutes were found together with membrane enzymes and proteins concerned with membrane structure. The proteins identified were categorised by their function and putative location on the basis of their homology with annotated proteins in other organisms.Keywords
This publication has 63 references indexed in Scilit:
- Dysferlin and the plasma membrane repair in muscular dystrophyPublished by Elsevier ,2004
- Inhibition of the Complement Membrane Attack Complex bySchistosoma mansoniParamyosinInfection and Immunity, 2003
- Molecular characterization and immunolocalization of Schistosoma mansoni ATP-diphosphohydrolaseBiochemical and Biophysical Research Communications, 2003
- Probability-based protein identification by searching sequence databases using mass spectrometry dataElectrophoresis, 1999
- Changes in cell morphology and actin organization during heat shock inDictyostelium discoideum: does HSP70 play a role in acquired thermotolerance?FEMS Microbiology Letters, 1999
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Functional and antigenic similarities between a 94-kD protein of Schistosoma mansoni (SCIP-1) and human CD59.The Journal of Experimental Medicine, 1994
- Immunity after treatment of human schistosomiasis mansoni: quantitative and qualitative antibody responses to tegumental membrane antigens prepared from adult wormsTransactions of the Royal Society of Tropical Medicine and Hygiene, 1987
- The formation and turnover of the membranocalyx on the tegument ofSchistosoma mansoniParasitology, 1977
- Schistosoma mansoni: Changes in the outer membrane of the tegument during development from cercaria to adult wormInternational Journal for Parasitology, 1973