STUDIES ON HUMAN CATARACTS .1. EVALUATION OF TECHNIQUES OF HUMAN CATARACT PRESERVATION AFTER EXTRACTION

  • 1 January 1981
    • journal article
    • research article
    • Vol. 20  (3) , 327-333
Abstract
The effects of several media and temperatures on the features (i.e., classification) of human cataracts were studied to define those storage conditions that least alter the appearance of the cataract after extraction. Distilled water, normal saline, TC 199 medium with extra bicarbonate and 5.5 mM glucose, and silicone oil at 4.degree., 26.degree. and 37.degree. C are all unsatisfactory for preserving cataracts for more than 1 h. Normal saline and TC 199 medium with bicarbonate and glucose are satisfactory for periods not exceeding 30-60 min at 4.degree. C. The best set of conditions, one that leads to no significant change in the appearance of the cataract, storage in a small covered glass vial at 4.degree. C with no added fluid led to no significant change in the cataract appearance. In this moist chamber no significant changes in cataract classification occurred during 4 h. The effect of -20.degree. C for 15 min on the appearance of a nuclear cataract was negligible. Lenses with this treatment are suitable for a correlation between the nuclear cataract''s CCRG [Cooperative Cataract Research Group] classification and its light scattering properties.

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