High Performance Liquid Chromatographic Determination of Pentamidine in Plasma
- 1 July 1986
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 9 (9) , 2035-2046
- https://doi.org/10.1080/01483918608078759
Abstract
A high performance liquid chromatographic method for quantitating pentamidine in plasma has been developed. Sample clean-up involved precipitating plasma with acetonitrile containing the internal standard, hexamidine. The supernatant was passed through a C8 Bond Elut column and eluted with a methanolic solution of sodium 1-heptanesulfonate. The eluate was then analyzed on an Altex C8 column with a mobile phase consisting of 45% CH8CN, 0.02% detramethylammonium chloride and 0.1% H3PO4. Using fluorescence detection (EX: 275 nm and EM: 340 nm), the detection limit was 1.25 ng/ml for 0.5 ml of plasma. The coefficients of variation for interday and intraday were around 10%.Keywords
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