The Bioassay of Vitamin B6 in Natural Materials

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Abstract
Vitamin B6 in various biological materials was determined with rats, using the modified vitamin B6-deficient ration (Sarma et al., '46) and the results compared with the values obtained by other methods. The values obtained are in most cases somewhat lower than the values obtained by the yeast growth method, presumably due to the decreased activity of pyridoxamine and pyridoxal when mixed in the ration. dl-Tryptophane, indole, dl-methionine, and oleic acid have been shown to exert a growth retarding effect when added to rations containing suboptimal amounts of pyridoxal or pyridoxine. This growth inhibition, however, was counteracted by adequate amounts of the vitamin. 1-Cystine and dl-alanine were shown to exert no appreciable effect under similar conditions. The possibility of these dietary factors influencing vitamin B6 estimation is discussed.