Calcium-binding protein in bull seminal vesicle secretion and seminal plasma

Abstract
A protein which showed high affinity for Ca ions was isolated from bull seminal vesicle secretion and seminal plasma. Its Ca-binding activity depended on the ionic strength and pH of the medium. The dissociation constant was 7.7 .times. 10-7 M and there were 14 binding sites/protein molecule. The MW of Ca-binding protein from bull seminal vesicle secretion, estimated by the gel filtration method, was 110,000. The protein may be involved in the regulation of the Ca ion level in seminal plasma.

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