Selective Medium forXanthomonas campestrispv.pruni

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Abstract
A simple selective medium (XPSM) was developed for the detection and isolation of X. campestris pv. (pathovar) pruni. The selectivity and sensitivity of X. campestris pv. pruni selective medium (XPSM) were evaluated using 9 X. campestris pv. pruni strains, 14 strains of 8 other Xanthomonas spp., and 1 strain each of Agrobacterium tumefaciens, Corynebacterium michiganense, Erwinia stewartii and Pseudomonas pseudoalcaligenes ssp. citrulli. The plating efficiencies of all X. campestris pv. pruni strains were the same or higher on XPSM than on nutrient agar supplemented with glucose. Two strains of X. campestris pv. begoniae and 1 strain of X. campestris pv. pelargonii grew on XPSM. However, the plating efficiencies of these strains on XPSM were generally low, and the colony appearance of these strains was clearly distinct from that of X. campestris pv. pruni. Colonies of 2 strains each of X. campestris pv. phaseoli, X. campestris pv. manihotis, X. campestris pv. vesicatoria and 1 strain of P. pseudoalcaligenes spp. citrulli appeared on XPSM, but growth was suppressed. No colonies of any of the other strains developed on XPSM. X. campestris pv. pruni virulence, sensitivity to lysis by 2 phages, and ability to produce characteristic yellow, mucoid colonies on nutrient agar supplemented with glucose were not detectably altered after growth on XPSM. Added X. campestris pv. pruni was quantitatively recovered from soil containing .apprx. 102-103 colony-forming units/g and was recovered from leaf extracts containing < 102-103 colony-forming units/ml. X. campestris pv. pruni was also readily detected in and isolated from extracts of lesions on naturally infected apricot leaves. Soil and leaf bacteria were generally suppressed on XPSM, and only an occasional fungal colony developed from soil samples on this medium.

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