Radiation Inactivation of Deoxyribonuclease in the Dry and Hydrated States

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Abstract
Dried deoxyribonuclease I adsorbed on cellulose, Celite, and Dowex 50 was more radiosensitive than the pure enzyme in the absence of adsorbents. The enzyme dried with DNA was protected from radiation-induced enzyme inactivation. Wet deoxyribonuclease, containing about 50% water, was less radiosensitive than dry enzyme. This suggests that bound water protects against radiation inactivation. The adsorbed enzyme was more radiosensitive when irradiated in air. The mechanisms responsible for these observed phenomena can be explained bv inter- or intramolecular energy transfer.