Characterization of digestive enzyme activity during larval development of gilthead seabream (Sparus aurata)
- 1 April 1996
- journal article
- Published by Springer Nature in Fish Physiology and Biochemistry
- Vol. 15 (2) , 121-130
- https://doi.org/10.1007/bf01875591
Abstract
The evolution of the digestive enzyme equipment in seabream from hatching to 30 days old larvae was studied; there was a progressive increase in the activity of protease, amylase and acid and alkaline phosphatase from day 15 onwards. The use of specific inhibitors, and SDS-PAGE provided evidence to suggest that most of the proteases belonged to the serine group. A high α-amylase activity was also denoted. Zymograms of larval extracts indicated that exogenous food has more a qualitative than a quantitative role in the secretion of digestive enzymes in this species.Keywords
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