PROTEIN METABOLISM OF RAT BRAIN SLICES

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Abstract
Rat brain slices respiring in a Krebs–Ringer phosphate medium were found to incorporate glycine-2-C14and DL-glutamic acid-1-C14into the protein.The addition of succinate as the substrate reduced the incorporation of glycine-2-C14into the protein of brain slices. The addition of glutamic acid, glutamine, and methionine sulphoxide into the glucose Krebs–Ringer phosphate medium inhibited the incorporation.The incorporation was also inhibited by 2,4-DNP in concentrations that do not decrease oxygen consumption.The respiration and the incorporation of glycine-2-C14were inhibited progressively with an increase in the concentration of isomytal, azacyclonol, chlorpromazine, and acetylpromazine. In the presence of succinate as the substrate these drugs did not inhibit the respiration of brain slices.

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