In-vitro binding of prostaglandin F-2 to uterine luminal proteins of pregnant and non-pregnant ewes

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Abstract
Pregnant and nonpregnant ewes were utilized to determine whether the presence of the embryo affected the binding of prostaglandin (PG) F2.alpha. to a uterine luminal protein. The uterine horn adjacent to the corpus luteum was flushed on day 13 of gestation or the estrous cycle. Flushings were incubated with [3H]PGF2.alpha. and subsequently eluted through a Sephadex colunm. Uterine luminal proteins of pregnant and non-pregnant ewes eluted with the void volume and failed to bind PGF2.alpha.