Separation of the Proteins of Locust Neurosecretory Endings with HPLC

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Abstract
High performance liquid chromatography [HPLC] on gel permeation columns was used to analyze the protein and peptide content of the neurosecretory corpora cardiaca of the locust (L. migratoria). A variety of extraction conditions and mobile phase compositions were tested for their separation efficiency. A good resolution, showing 12 peaks distributed within 5 distinct groups, could be achieved in 32 min with the fresh homogenate from only 2 corpora cardiaca. Similar HPLC patterns were obtained with 3 fractions previously separated on a Sephadex G-100 column as well as with isolated neurosecretory vesicles. HPLC then appears as a promising technique in the field of insect neurohormone isolation.