GLYCYL-TRANSFER AND ALANYL-TRANSFER RNA-SYNTHETASES FROM BOMBYX-MORI - PURIFICATION AND PROPERTIES
- 1 January 1984
- journal article
- research article
- Vol. 259 (7) , 4043-4048
Abstract
Glycyl- and alanyl-tRNA synthetases were purified from an extract of B. mori posterior silk glands by a procedure that allows for the simultaneous isolation of both enzymes. Glycyl-tRNA synthetase is a dimer of MW = 160,000 consisting of similar or identical subunits; alanyl-tRNA synthetase is a monomer of MW = 110,000-115,000. The abundance of these 2 enzymes in the posterior silk gland is consistent with the observed adaptation of this organ to the production of the silk protein, fibroin. The 2 enzymes are similar in oligomeric structure to the corresponding enzymes in Saccharomyces cerevisiae, but dissimilar from their counterparts in Escherichia coli.This publication has 4 references indexed in Scilit:
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