Induction of error-prone repair as a consequence of DNA ligase deficiency in Escherichia coli.
- 1 November 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (11) , 4645-4649
- https://doi.org/10.1073/pnas.72.11.4645
Abstract
DNA ligase deficiency is shown to induce generalized mutator activity in E. coli. This mutator activity is unaffected by 3 mug/ml of chloramphenicol but is abolished both in lig-recA double mutants and by incubation with 20 mug/ml of chloramphenicol. Dna ligase deficiency is also shown to reactivate ultraviolet light-irradiated phage lambda and T7 and to increase both spontaneous and ultraviolet light-induced mutagenesis in phage lambda, all of which are abolished in lig-recA strains. Interaction occurs between the molecular events of mutagenesis induced by ultraviolet irradiation and those induced by DNA ligase deficiency. These observations suggest a common pathway, coordinately expressed with the inducible repair mode, that is responsible for mutagenesis.Keywords
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