Physicochemical Properties of Deoxyribonucleic Acid from an Extreme Thermophile

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Abstract
Deoxyribonucleic acid was extracted from an extremely thermophilic bacterium and its physicochemical properties were investigated. The melting temperature in 0.15 m sodium chloride, that in 0.015 m sodium chloride, and the density in cesium chloride were, respectively, 97.5°C, 81.3°C, and 1.727. The guanine plus cytosine content of the DNA was estimated to be 68% from these values, and this is in good accord with the GC content found by acid hydrolysis. The circular dichroic spectrum suggested that the nucleic acid contains base pairs with a different conformation from the ordinary B form. No significant difference was observed between the priming activities of thermophile and mesophile DNA's for mesophile RNA pdv-merase [EC 2.7.7.6].

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