Sensitivity to Bacillus subtilis: A Novel System for Selection of Heterozygous Diploids of Dictyostelium discoideum

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Abstract
The initial postulate that drug-sensitive mutations used in pairs might enable diploids to be selected, was shown by the isolation of doubly resistant diploids on plates containing methanol and cycloheximide. The isolates were all slow-growing strains and not good parental stocks. The use of haploids bearing the bsg marker together with tsg bearing haploids has several advantages including low reversion rates, diploid clones are recognizable early and Bsg strains are wild type in regard to temperature response. The bsg marker can be used to perform a number of genetic manipulations which are otherwise difficult or impossible.