Evidence for two closely related isozymes of arylamine N‐acetyltransferase in human liver
- 13 February 1989
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 244 (1) , 203-207
- https://doi.org/10.1016/0014-5793(89)81193-7
Abstract
Acetyl CoA‐dependent arylamine N‐acetyltransferase (EC 2.3.1.5) is the target of a genetic polymorphism in the metabolism of drugs and carcinogens. N‐Acetyltransferase was purified 1000‐fold from cytosol of human liver and its identity was verified by amino acid sequence homology of two of its tryptic peptides with published rabbit and chicken N‐acetyltransferase sequences. Enzyme activity correlated with the presence of two proteins, NAT‐1 and NAT‐2, with indistinguishable molecular masses (31 kDa). NAT‐1 and NAT‐2 could be separated by anion‐exchange chromatography and were functionally distinguished by their different apparent affinities for the acceptor amine sulfamethazine (SMZ). Antibodies raised against NAT‐1 were able to recognize both isozymes on Western blots.Keywords
This publication has 18 references indexed in Scilit:
- A new siliconized‐glass fiber as support for protein‐chemical analysis of electroblotted proteins.European Journal of Biochemistry, 1988
- Characterization of the common genetic defect in humans deficient in debrisoquine metabolismNature, 1988
- Genetic polymorphism of human cytochrome P-450 (S)-mephenytoin 4-hydroxylase. Studies with human autoantibodies suggest a functionally altered cytochrome P-450 isozyme as cause of the genetic deficiencyBiochemistry, 1987
- A two-step procedure for efficient electrotransfer of both high-molecular-weight (>400,000) and low-molecular-weight (<20,000) proteinsAnalytical Biochemistry, 1987
- Microscale isocratic separation of phenylthiohydantoin amino acid derivativesJournal of Chromatography A, 1985
- Purification and physical-chemical properties of acetyl-CoA: Arylamine N-acetyltransferase from pigeon liverBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1983
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Genetic Control of Isoniazid Metabolism in ManBMJ, 1960
- On the Evaluation of the Constants
V
m
and
K
M
in Enzyme ReactionsScience, 1952