Optimizing Recovery of Platelets in Platelet Rich Plasma by the Simplex Strategy

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Abstract
A Simplex is an empirical, geometric feedback strategy which progressively programs a series of experiments acccording to the results of the preceeding experiments. It leads to definition of conditions which maximize or minimize the result of experiments in 2 or more variables. When applied to study a laboratory method, it dictates simultaneous variation of the conditions of an experiment, differing from the usual approach in which variables are altered sequentially and arbitrarily. A 2 variable Simplex (force and duration of centrifugation) was applied to maximize platelet recovery in platelet rich plasma (PRP). The technique of Simplex is described. Maximal recovery of platelets in PRP was reached by centrifugation at 2160 .times. g for 2.7 min. To confirm the correctness of this result, platelet concentrates were prepared from PRP''s separated by 3 different methods: conditions determined by Simplex, American National Red Cross recommended method (2820 .times. g for 2 min) and Canadian Red Cross recommended method (995 .times. g for 9 min). Mean recovery of 78% was obtained by Simplex, significantly higher than 61% with the American National Red Cross method (P > .005) and higher than 69% with the Canadaian Red Cross method. Simplex strategy is recommended as a simple, powerful technique for determining optimal experimental or laboratory conditions.

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