FC RECEPTORS FOR IGG, IGM AND IGE ON HUMAN LEUKEMIC LYMPHOCYTES

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 35  (2) , 286-295
Abstract
Fc receptors for Ig[immunoglobulin]G, IgM, IgE and the cell surface immunoglobulins (SIg) were analyzed on lymphocytes from 17 patients with chronic lymphatic leukemia (CLL), 1 with lymphosarcoma cell leukemia (LSL) and 2 each with hairy cell leukemia (HCL), acute lymphatic leukemia (ALL) and Sezary syndrome. Fc receptors for IgG and IgM were detected by rosette formation with ox erythrocytes (EO) sensitized with rabbit IgG (EOAG) and IgM (EOAM) anti-EO antibodies [Ab], respectively. Fc receptors for IgE were analyzed with EO coated with glutaraldehyde coupled complexes consisting of rabbit Fab'' fragments of anti-EO Ab and Fc fragments of an IgE myeloma protein (EOAE), or with aldehyde fixed EO to which IgE was adsorbed. SIg of classes IgM, IgD, IgG and .kappa. and .lambda. L chain type were detected with EO coated with complexes consisting of Fab''-anti-EO and purified F(ab'')2 fragments of specific goat Ab. Lymphocytes of all patients with CLL, LSL and HCL had Fc receptors for IgG (65 .+-. 15% EOAG+, normal 22.0 .+-. 5.8%). Ten patients had significant numbers of cells with IgM Fc receptors (37 .+-. 22% EOAM+, normal 1.2 .+-. 1.5%) which were detected without overnight culturing of the lymphocytes. Lymphocytes of 4 patients (2 CLL, 1 LSL, 1 HCL) had Fc receptors for IgE (22-88% EOAE+, normal 1.8 .+-. 0.7%). The cells of 3 of these 4 patients were EOAM+. The high numbers of rosetting cells indicated that individual lymphocytes must have carried more than 1 class of Fc receptors. The lymphocytes of the ALL and Sezary syndrome patients had few Fc receptor positive cells. Of the 17 patients with CLL, 12 were SIgM+ and/or SIgD+, only 4 .kappa.+ or .lambda.+ and 1 had no SIg. The cells of the LSL and 1 of the HCL patients were SIgM+ and SIgD+, while the cells of the other HCL patient were SIgG, .lambda.+. None of the other patients had more than 10% SIgG+ cells. The ALL and Sezary syndrome patients had low numbers of SIg+ and Fc receptor positive cells. Lymphocytes of patients with B[bone marrow-derived]-cell leukemias may carry different classes of Fc receptors simultaneously; the different classes are found in a decreasing frequency of IgG > IgM > IgE.