Unusual conformation of (dA)n·(dT)n-tracts as revealed by cyclobutane thymine – thymine dimer formation
Open Access
- 1 January 1991
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 19 (16) , 4491-4496
- https://doi.org/10.1093/nar/19.16.4491
Abstract
Cyclobutane dimer formation has been used to probe conformation of (dA)n·(dT)n-tracts cloned in plasmid DNA. The observed dimer probability patterns for (dA)n·(dT)n -tracts with n ≥ 4 exhibit maximum intensity at the 3′-terminal TT site of Tn-tract, whereas photoreactivity at all the other TT sites is inhibited. Both temperature and dimethyl sulfoxide increase dimer formation within Tn-tracts and result in an even dimer pattern. The data obtained have been interpreted in terms of an unusual structure adopted by (dA)n·(dT)n -tracts. An influence of flanking base pairs, ethidium bromide binding and ionic strength has also been studied.Keywords
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