THE EFFECT OF D-C-PROPARGYLGLYCINE AND D-C-ALLYLGLYCINE ON RATS WITH SPECIAL REFERENCE TO D-AMINO ACID OXIDASE

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Abstract
I.p. injection of D-C-propargylglycine, a potent suicide substrate for D-amino acid oxidase (EC 1.4.3.3), to rats resulted in remarkable glucosuria and aminoaciduria. Glucosuria continued for about 1 wk after the injection and then returned to normal level. D-C-propargylglycine also appeared in the urine, but only for several hours at the beginning. Almost all the amino acids detectable by amino acid analysis increased in the urine after the injection, and lysine, glycine and alanine were predominant. D-Amino acid oxidase activity of the kidney and liver decreased to about 80% that of control rats at 24 h after the injection. L-C-propargylglycine injection did not produce any glucosuria or amino aciduria. The injection of D-C-allylglycine into rats also resulted in glucosuria, but the injection of L-C-allylglycine did not. A relatively large dose of D- and L-C-propargylglycine caused convulsions in rats. The D-configuration and the unsaturated bond of these 2 model substrates probably produce some metabolic disorder in the tissues, in which D-amino acid oxidase is possible involved. Convulsions and the urinary changes may be produced by 2 independent mechanisms.