Multipotential Electrochemical Detection of Primer Extension Reactions on DNA Self-Assembled Monolayers

Abstract
Electroactive nucleoside triphosphates (“electrotides”) have been incorporated into primers by DNA polymerase and detected on oligonucleotide surface-assembled monolayers. Four electrotides bearing three different electroactive moietiesferrocene, vinylferrocene, and anthraquinoneare detected in four alternative formats.