Verification of Multichannel Liquid Dispenser Performance in the 4–30 μL Range by Using Optical Pathlength Measurements in Microplates

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Abstract
Pipettors and liquid handling systems should be checked regularly to verify their precision and accuracy. Professional organizations recommend performing 4-replicate tests monthly and 10-replicate tests at least quarterly (1)(2)(3)(4). Traditional gravimetric procedures are adequate to meet these testing requirements for single-channel pipettors but are tedious and impractical for multichannel devices. An alternative is to dispense a solution of colored dye into wells of a microplate and to measure the resulting absorbance values in a microplate reader. Volume calibration is performed with a calibration curve relating absorbance to volume (5).

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