New PCR assay for rapid and quantitative detection of human cytomegalovirus in cerebrospinal fluid
- 1 February 1996
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 34 (2) , 482-483
- https://doi.org/10.1128/jcm.34.2.482-483.1996
Abstract
Rapid Chelex extraction combined with an automated hybridization assay for the detection of PCR-amplified human cytomegalovirus DNA from cerebrospinal fluid was established. Quantitation of DNA was performed with a plasmid being used as an external standard. The detection limit was 10 copies per microliter. Quantitative detection of human cytomegalovirus DNA could be achieved over a range from 10 to 10(4) copies per microliter.Keywords
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