Some characteristics of early appearing isomaltase in intestinal mucosa of suckling rat.
- 1 January 1979
- journal article
- research article
- Published by Center for Academic Publications Japan in Journal of Nutritional Science and Vitaminology
- Vol. 25 (6) , 543-552
- https://doi.org/10.3177/jnsv.25.543
Abstract
Characteristics of early appearing free isomaltase in the soluble fraction were investigated in rat intestinal mucosa. Soluble isomaltase and membrane-bound sucrase-isomaltase complex were prepared from 15 day old rat intestine. Immunochemical properties, optimal pH and heat sensitivity of soluble isomaltase were compared with those of membrane-bound isomaltase. Optimal pH of free isomaltase in the soluble fraction was lower than that of membrane-bound isomaltase. Soluble and membrane-bound isomaltase showed different sensitivities for temperature. Membrane-bound isomaltase in 15 day old suckling rat intestine gave a single line with antiserum; soluble isomaltase gave no precipitin line. Soluble isomaltase is not derived from the isomaltase moiety of membrane-bound sucrase-isomaltase complex as a result of mechanical fragility and rather it would be lysosomal in origin.This publication has 9 references indexed in Scilit:
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