Plant regeneration from callus tissue cultures of the cherry rootstock Colt (Prunus aviumxP. pseudocerasus) and the apple rootstock M.25 (Malus pumita)

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Abstract
Callus was initiated in vitro from the roots of intact micropropagated plantlets of the cherry rootstock Colt and the apple rootstock M.25, and also from the excised roots of such plantlets. With Colt, the callus from the roots of the intact plantlets regenerated shoots and embryoid-like structures, whereas callus from the excised roots regenerated only shoots. There was also some shoot regeneration with M.25 but this was at a lower frequency and was confined to the callus from the roots of the intact plantlets. There were no embryoid-like structures with M.25 callus. Shoots originating directly from the callus or from the embryoid-like structures have been micropropagated to produce plants for evaluation in the field. Thus far, these plants appear to be true to type.

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