Analysis of the promotors of the single‐copy genes for plastocyanin and subunit δ of the chloroplast ATP synthase from spinach

Publisher Website
Google Scholar
Abstract
The promotors of the single‐copy genes for subunit δ of the chloroplast ATP synthase (atpD) and plastocyanin (PC) from spinach have been sequenced, dissected and analysed in transgenic F0 and F1 tobacco plants using the bacterial GUS gene as a reporter for promotor activity. The transcription of these genes is photo‐controlled. The results have been compared with those obtained for the spinach rbcS‐1 gene, one of the light‐regulated genes encoding the small subunit of ribulose‐1,5‐bisphosphate carboxylase/oxygenase, and for the cauliflower mosaic virus (CaMV) 35S RNA promotor. We find that the 5′ upstream regions of about 1200 nucleotides contain all the sequences required for light regulation, organ‐, tissue‐ and development‐specific expression, and that they are structurally diverse. Their cis‐acting elements are functionally defined. The proximal regions of the spinach promotors contain potential TATA, CAAT and T‐cyt boxes at appropriate positions, but only sequence elements with low similarity to published light‐responsive elements. Positive light‐stimulated regions, regions with constitutive, light‐independent enhancing effects and with ‘silencer’‐like activity in complete darkness are found in proximal and far upstream promotor segments. Highest activity of these promotors is correlated with the presence of chloroplasts but is not confined to photosynthetic tissue. Surprisingly, expression is observed in the phloem regions of transgenic leaves, leaf and floral stems, in the vascular area of anthers and in pollen. No histochemical staining has been detected in roots. The distal region of atpD located between −1137 and −590 contains elements for expression in the outer phloem, the region from −590 to −185 for activity in the inner phloem of floral stems. Similar tissue‐specific patterns are observed with a fusion between the caufliflower mosaic virus 35S RNA promotor and the GUS gene.